Advanced Andrology + Cryo Biology Course

Course 3 – Advanced Andrology & Cryobiology
Duration: 6 days
Fees: INR 50000/-

• Introduction – Spermatogenesis
• Semen Sample collection
• Safe Gamete Handling and good laboratory practice in Andrology
• Basic Microscopic principles and techniques in Semen Analysis
• Semen Analysis WHO 2010 criteria
• Physical Parameters of semen
  • Volume, colour, odour
  • Ph
  • Viscosity
• Microscopical parameters of semen
  • Sperm Count (Maklar Chamber)
  • Sperm Motility
  • Cryptozoospermia & Azoospermia
  • Sperm Morphology WHO 2010
  • Sperm Vitality Assay
    • Staining
    • HOST
• Terminology in Andrology
• Interpretation of Semen analysis report
• Sperm DNA Fragmentation

Sperm Preparation Techniques

• Double layer density gradient
• Swim up

• Processing PESA/TESA sample
• Preparation of infected semen sample

Cryobiology

• Sperm freezing of processed sampl
• Sperm freezing of raw semen sample
• Sperm Thawing

Quality Assurance in Andrology
Discussion
Q & A